Article
tRNA2Thr complements temperature sensitivity caused by null mutations in the htrB gene in Escherichia coli.
Journal of bacteriology - 1 Mar 2003
Mohri Yoshio, Goto Simon, Nakahigashi Kenji, Inokuchi Hachiro
Abstract excerpt
According to the wobble rule, tRNA2Thr is nonessential for protein synthesis, because the codon (ACG) that is recognized by tRNA2Thr is also recognized by tRNA4Thr. In order to investigate the reason that this nonessential tRNA nevertheless exists in Escherichia coli, we attempted to isolate tRNA2Thr-requiring mutants. Using strain JM101F(-), which lacks the gene for tRNA2Thr, we succeeded in isolating two...
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