Construction and characterization of an Escherichia coli mutant deficient in the metY gene encoding tRNA(f2Met): either tRNA(f1Met) or tRNA(f2Met) is required for cell growth.

doi:10.1016/0378-1119(92)90715-2

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Article

Construction and characterization of an Escherichia coli mutant deficient in the metY gene encoding tRNA(f2Met): either tRNA(f1Met) or tRNA(f2Met) is required for cell growth.

Gene - 1 May 1992

Kenri T, Imamoto F, Kano Y

Abstract excerpt

The Escherichia coli metY gene, encoding tRNA(f2Met), was split by the kanamycin-resistance-encoding gene. The resulting mutant exhibited the same growth rate as the wild type, indicating that tRNA(f2Met) is not indispensable as is the case with the metZ gene encoding tRNA(f1Met) [Kenri et al., G...

Topics

Blotting, Southern
Codon
DNA, Recombinant
Escherichia coli
Kanamycin Resistance
Mutagenesis, Insertional
Mutation
Plasmids
RNA, Bacterial
RNA, Transfer, Met
Recombinant Fusion Proteins
beta-Galactosidase

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