The lethal phenotype caused by null mutations in the Escherichia coli htrB gene is suppressed by mutations in the accBC operon, encoding two subunits of acetyl coenzyme A carboxylase.

Abstract excerpt

Insertion mutations in the Escherichia coli htrB gene result in the unique phenotype of not affecting growth at temperatures below 32.5 degrees C but leading to a loss of viability at temperatures above this in rich media. When htrB bacteria growing in rich media were shifted to the nonpermissive temperature of 42 degrees C, they continued to grow at a rate similar to that at 30 degrees C but they produced...

Topics

• Acetyl-CoA Carboxylase
• Base Sequence
• Cloning, Molecular
• Escherichia coli
• Genes, Bacterial
• Genes, Lethal
• Genes, Suppressor
• Genetic Complementation Test
• Kinetics
• Lipopolysaccharides