Article
Investigation of the stability of yeast rad52 mutant proteins uncovers post-translational and transcriptional regulation of Rad52p.
Genetics - 1 Jan 2003
Asleson Erin N, Livingston Dennis M
Abstract excerpt
We investigated the stability of the Saccharomyces cerevisiae Rad52 protein to learn how a cell controls its quantity and longevity. We measured the cellular levels of wild-type and mutant forms of Rad52p when expressed from the RAD52 promoter and the half-lives of the various forms of Rad52p when expressed from the GAL1 promoter. The wild-type protein has a half-life of 15 min. rad52 mutations variably affect...
Topics
- Cysteine Endopeptidases
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Multienzyme Complexes
- Mutation
- Proteasome Endopeptidase Complex
- Protein Processing, Post-Translational
- RNA, Messenger
- Rad51 Recombinase
- Rad52 DNA Repair and Recombination Protein
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins