Article
A co-localization assay for the analysis of protein-protein interactions.
Gene - 15 Feb 2007
Köhler Fabian
Abstract excerpt
The understanding and analysis of protein associations in living cells is a major goal of molecular biology. Here, we describe an assay for the analysis of protein-protein interactions based on the co-localization of a fused site-specific protease with a cleavable reporter in close proximity to the interaction partner under examination. We exemplified this scheme in the temperature-sensitive Saccharomyces...
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