Article
Biotinylated theta-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells.
Journal of lipid research - 1 May 2003
Sugii Shigeki, Reid Patrick C, Ohgami Nobutaka, Shimada Yukiko, Maue Robert A, Ninomiya Haruaki, Ohno-Iwashita Yoshiko, Chang Ta-Yuan
Abstract excerpt
BCtheta is a proteolytically nicked and biotinylated derivative of a cholesterol binding protein perfringolysin O (theta-toxin), and has been used to detect cholesterol-rich domains at the plasma membrane (PM). Here we show that by modifying the cell fixation condition, BCtheta can also be used to detect cholesterol-rich domains intracellularly. When cells were processed for PM cholesterol staining, the...
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