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Article

Membrane translocation assay based on proteolytic cleavage: application to diphtheria toxin T domain.

Biochimica et biophysica acta - 1 Jan 2015

Rodnin Mykola V, Ladokhin Alexey S

Abstract excerpt

The function of diphtheria toxin translocation (T) domain is to transfer the catalytic domain across the endosomal membrane upon acidification. The goal of this study was to develop and apply an in vitro functional assay for T domain activity, suitable for investigation of structure-function relationships of translocation across lipid bilayers of various compositions. Traditionally, T domain activity in vitro is...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 25291602
DOI: 10.1016/j.bbamem.2014.09.013
PMCID: PMC4259893

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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