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Article

Molecular analysis and characterization of a broad-host-range plasmid, pEP2.

Journal of bacteriology - 1 Sept 1994

Zhang Y, Praszkier J, Hodgson A, Pittard A J

Abstract excerpt

Plasmid pEP2 was found to encode a protein, RepA, which is essential and rate limiting for its replication in Escherichia coli and Corynebacterium pseudotuberculosis. Mutations which altered the rate of synthesis of this protein in E. coli affected the copy number and segregational stability of pEP2 in the two hosts. RepA contains 483 amino acid residues and has the calculated molecular weight of 53,925. It shows...

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Identifiers

PMID: 7521871
DOI: 10.1128/jb.176.18.5718-5728.1994
PMCID: PMC196776

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