Article
Molecular analysis and characterization of a broad-host-range plasmid, pEP2.
Journal of bacteriology - 1 Sept 1994
Zhang Y, Praszkier J, Hodgson A, Pittard A J
Abstract excerpt
Plasmid pEP2 was found to encode a protein, RepA, which is essential and rate limiting for its replication in Escherichia coli and Corynebacterium pseudotuberculosis. Mutations which altered the rate of synthesis of this protein in E. coli affected the copy number and segregational stability of p...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Corynebacterium pseudotuberculosis
- DNA Helicases
- DNA Replication
- DNA, Recombinant
- DNA, Single-Stranded
- DNA-Binding Proteins
- Escherichia coli
- Gene Amplification
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Plasmids
- Protein Biosynthesis
- Proteins
- RNA, Bacterial