Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli.

Journal of molecular biology - 14 Feb 2003

Laursen Brian Søgaard, Siwanowicz Igor, Larigauderie Guilhem, Hedegaard Jakob, Ito Koreaki, Nakamura Yoshikazu, Kenney John M, Mortensen Kim Kusk, Sperling-Petersen Hans Uffe

Abstract excerpt

The infB gene encodes translation initiation factor IF2. We have determined the entire sequence of infB from two cold-sensitive Escherichia coli strains IQ489 and IQ490. These two strains have been isolated as suppressor strains for the temperature-sensitive secretion mutation secY24. The mutations causing the suppression phenotype are located within infB. The only variations from the wild-type (wt) infB found in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Circular Dichroism
Share to Cold Temperature

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Complementation Test

Share to GTP Phosphohydrolases

Share to Guanosine Triphosphate

Share to In Vitro Techniques

Share to Protein Engineering

Share to Restriction Mapping

Source

Identifiers

PMID: 12559921
DOI: 10.1016/s0022-2836(02)01367-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli.PMID 12559921Read ↗

Mutation of Thr445 and Ile500 of initiation factor 2 G-domain affects Escherichia coli growth rate at low temperature.Semantic proximity Read ↗

In vivo study of engineered G-domain mutants of Escherichia coli translation initiation factor IF2.Semantic proximity Read ↗

A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli.Semantic proximity Read ↗

Weakening the IF2-fMet-tRNA Interaction Suppresses the Lethal Phenotype Caused by GTPase Inactivation.Semantic proximity Read ↗

Translation initiation without IF2-dependent GTP hydrolysis.Semantic proximity Read ↗

Initiation factor 2 of Myxococcus xanthus, a large version of prokaryotic translation initiation factor 2.Semantic proximity Read ↗

In vitro study of two dominant inhibitory GTPase mutants of Escherichia coli translation initiation factor IF2. Direct evidence that GTP hydrolysis is necessary for factor recycling.Semantic proximity Read ↗

Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.Semantic proximity Read ↗

Disparate Phenotypes Resulting from Mutations of a Single Histidine in Switch II of Geobacillus stearothermophilus Translation Initiation Factor IF2.Semantic proximity Read ↗

Molecular characterization of the essential response regulator protein YycF in Bacillus subtilis.Semantic proximity Read ↗

Mutations in 16S rRNA that suppress cold-sensitive initiation factor 1 affect ribosomal subunit association.Semantic proximity Read ↗

Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.