Article
In vivo study of engineered G-domain mutants of Escherichia coli translation initiation factor IF2.
Molecular microbiology - 1 Jan 1994
Laalami S, Timofeev A V, Putzer H, Leautey J, Grunberg-Manago M
Abstract excerpt
During the IF2-catalysed formation of the 30S initiation complex, the GTP requirement and its subsequent hydrolysis during 70S complex formation are considered to be essential for translation initiation in Escherichia coli. In order to clarify the role of certain amino acid residues believed to b...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Escherichia coli
- Genes, Bacterial
- Genotype
- Guanosine Triphosphate
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Peptide Initiation Factors
- Plasmids
- Point Mutation
- Prokaryotic Initiation Factor-2
- Protein Engineering
- Protein Structure, Secondary