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Article

In vivo study of engineered G-domain mutants of Escherichia coli translation initiation factor IF2.

Molecular microbiology - 1 Jan 1994

Laalami S, Timofeev A V, Putzer H, Leautey J, Grunberg-Manago M

Abstract excerpt

During the IF2-catalysed formation of the 30S initiation complex, the GTP requirement and its subsequent hydrolysis during 70S complex formation are considered to be essential for translation initiation in Escherichia coli. In order to clarify the role of certain amino acid residues believed to be crucial for the GTP hydrolytic activity of E. coli IF2, we have introduced seven single amino acid substitutions into...

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PMID: 8170391
DOI: 10.1111/j.1365-2958.1994.tb00309.x

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In vivo study of engineered G-domain mutants of Escherichia coli translation initiation factor IF2.PMID 8170391Read ↗

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