Article
A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli.
Journal of molecular biology - 20 Jul 1991
Laalami S, Putzer H, Plumbridge J A, Grunberg-Manago M
Abstract excerpt
We have constructed strains carrying null mutations in the chromosomal copy of the gene for translational initiation factor (IF) 2 (infB). A functional copy of the infB gene is supplied in trans by a thermosensitive lysogenic lambda phage integrated at att lambda. These strains enabled us to test in vivo the importance of different structural elements of IF2 expressed from genetically engineered plasmid...
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