Article
A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli.
Journal of molecular biology - 20 Jul 1991
Laalami S, Putzer H, Plumbridge J A, Grunberg-Manago M
Abstract excerpt
We have constructed strains carrying null mutations in the chromosomal copy of the gene for translational initiation factor (IF) 2 (infB). A functional copy of the infB gene is supplied in trans by a thermosensitive lysogenic lambda phage integrated at att lambda. These strains enabled us to test...
Topics
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Chromosome Deletion
- Chromosomes, Bacterial
- Codon
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes
- Peptide Initiation Factors
- Plasmids
- Prokaryotic Initiation Factor-2