Article
[HLA genotyping by automatic semi-quantitative polymerase chain reaction-reverse sequence specific oligonucleotide].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Jan 2003
Xiao Lu-lu, Hao Gui-qin, Ye Xin, Chen Hong-tao, Tan Yin, Zhang Sheng
Abstract excerpt
OBJECTIVE: To explore a HLA genotyping method that can be used for organ transplantation tissue typing, and especially for establishing hemopoietic stem cell bank and the cord blood stem cell bank by analyzing the PCR-DNA. METHODS: Modified automatic method based on semi-quantitative amplification system of HLA-I, I allele genotyping was established using reverse sequence-specific oligonucleotide with polymerase...
Topics
- Alleles
- DNA
- Genotype
- Histocompatibility Antigens Class I
- Histocompatibility Antigens Class II
- Polymerase Chain Reaction
