Article
Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR sequence specific primers.
Genetic testing - 1 Jan 2004
Downing J, Guttridge M G, Thompson J, Darke C
Abstract excerpt
We have developed a strategy for five-locus human leukocyte antigen (HLA) typing of hematopoeitic stem cell (HSC) donors using the polymerase chain reaction with sequence-specific primers (PCR-SSP). The PCR-SSP method is robust, reproducible, and accurate. New PCR-SSP mixtures can be added as required and all reactions are carried out under the same conditions, which can easily be applied to the typing of other...
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