Article
Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR sequence specific primers.
Genetic testing - 1 Jan 2004
Downing J, Guttridge M G, Thompson J, Darke C
Abstract excerpt
We have developed a strategy for five-locus human leukocyte antigen (HLA) typing of hematopoeitic stem cell (HSC) donors using the polymerase chain reaction with sequence-specific primers (PCR-SSP). The PCR-SSP method is robust, reproducible, and accurate. New PCR-SSP mixtures can be added as req...
Topics
- Alleles
- Base Sequence
- DNA Primers
- DNA Probes, HLA
- Female
- Genotype
- HLA Antigens
- HLA-DR Antigens
- HLA-DRB1 Chains
- Hematopoietic Stem Cell Transplantation
- Histocompatibility Testing
- Humans
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Tissue Donors