[High through-put genomic DNA isolation technique and its application in HLA genotyping for samples from bone marrow donor program].

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[High through-put genomic DNA isolation technique and its application in HLA genotyping for samples from bone marrow donor program].

Zhongguo shi yan xue ye xue za zhi - 1 Oct 2009

Wang Da-Ming, Tang Si, Li Zhen, Cheng Xi, Gao Su-Qing, Deng Zhi-Hui

Abstract excerpt

This study was aimed to develop and establish an efficient method for high through-put automatically extracting genomic DNA from EDTA-anticoagulated whole blood samples, and to utilize this method in routine rSSO HLA genotyping by luminex flow array assay, the genomic DNA was extracted automatically from 400 microl blood samples by using TECAN DNA workstation and 96-well plate with 2 ml volume per well. The yield...

Topics

Biological Specimen Banks
Bone Marrow
DNA
DNA Primers
Genotype
HLA Antigens
High-Throughput Screening Assays
Humans
Living Donors
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis

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Open in PubMed

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PMID: 19840464

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