Article
[DNA typing for HLA-A by polymerase chain reaction with sequence-specific primers].
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao - 1 Jun 1999
Tan J, Tang X
Abstract excerpt
The authors have established a method of DNA typing for HLA-A antigen in Chinese by using polymerase chain reaction with sequence-specific primers (PCR-SSP). HLA-A alleles were successfully typed in all clinical samples and 36 standard DNAs by PCR-SSP. A total of 635 A antigens were typed in 345 donor-recipients, including 55 homozygotes and 290 heterozygotes. Twenty alleles of HLA-A were accurately distinguished...
Topics
- Alleles
- Asian People
- DNA
- DNA Primers
- HLA-A Antigens
- Histocompatibility Testing
- Humans
- Kidney Transplantation
- Polymerase Chain Reaction
