Article
An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl-N- nitrosourea-mutagenized mouse embryonic stem cells.
Proceedings of the National Academy of Sciences of the United States of America - 26 Nov 2002
Vivian Jay L, Chen Yijing, Yee Della, Schneider Elizabeth, Magnuson Terry
Abstract excerpt
Using selectable genes as proof of principle, a new high-throughput genotype-based mutation screen in mouse embryonic stem (ES) cells was developed [Chen et al. (2002) Nat. Genet. 24, 314-317]. If expanded to nonselectable genes, this approach would allow one to proceed quickly from sequence to whole-animal phenotypes. Here data are presented showing that a screen of a cryopreserved library of clonal, germ line...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.