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Article

Rapid phenotypic assay for human immunodeficiency virus type 1 protease using in vitro translation.

Journal of virological methods - 1 Oct 2002

Iga Mutsunori, Matsuda Zene, Okayama Akihiko, Sugiura Wataru, Hashida Seiichi, Morishita Kazuhiro, Nagai Yoshiyuki, Tsubouchi Hirohito

Abstract excerpt

A rapid in vitro phenotyping method for human immunodeficiency virus type 1 (HIV-1) protease was developed. In this system, both HIV-1 protease and substrates are prepared using a rabbit reticulocyte based coupled in vitro transcription/translation system. The activity of protease is evaluated by the amount of cleaved substrate measured by ELISA. In this system, wild-type protease derived from strain HXB2 was...

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Identifiers

PMID: 12367727
DOI: 10.1016/s0166-0934(02)00133-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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