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Article

Rapid enzymatic test for phenotypic HIV protease drug resistance.

Biological chemistry - 1 Jul 2003

Hoffmann Dieter, Assfalg-Machleidt Irmgard, Nitschko Hans, von der Helm Klaus, Koszinowski Ulrich, Machleidt Werner

Abstract excerpt

A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid one-step procedure as active enzyme and tested for inhibition by five selected synthetic inhibitors (amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir) used presently for chemotherapy of HIV-infected patients. The HPLC system...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 12956428
DOI: 10.1515/BC.2003.124

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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