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Design, production, and characterization of a monomeric streptavidin and its application for affinity purification of biotinylated proteins.

Protein expression and purification - 1 Aug 2002

Qureshi Mohammad Hassan, Wong Sui-Lam

Abstract excerpt

To expand the application of the streptavidin-biotin technology for reversible affinity purification of biotinylated proteins, a novel form of monomeric streptavidin was engineered and produced using Bacillus subtilis as the expression host. By changing as little as two amino acid residues (T90 and D128) to alanine, the resulting mutant streptavidin designated DM3 was produced 100% in the monomeric form as a...

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PMID: 12182820
DOI: 10.1016/s1046-5928(02)00021-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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