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Article

Superactive SecY variants that fulfill the essential translocation function with a reduced cellular quantity.

The Journal of biological chemistry - 13 Dec 2002

Mori Hiroyuki, Shimizu Yuhsuke, Ito Koreaki

Abstract excerpt

The fifth and the sixth cytoplasmic regions (C5 and C6) of SecY are important for the SecA-driven preprotein translocation reaction. A cold-sensitive mutation, secY205 (Tyr-429 --> Asp), in C6 impairs the ATP- and precursor-dependent SecA insertion into the membrane. We now identified second site mutations that suppressed the defect. Cis-placement of these mutations proved to suppress mutations at another...

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Identifiers

PMID: 12351621
DOI: 10.1074/jbc.M204436200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Superactive SecY variants that fulfill the essential translocation function with a reduced cellular quantity.PMID 12351621Read ↗

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Genetic dissection of SecA: suppressor mutations against the secY205 translocase defect.Semantic proximity Read ↗

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