Article
An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY.
Proceedings of the National Academy of Sciences of the United States of America - 24 Apr 2001
Mori H, Ito K
Abstract excerpt
The SecY/Sec61alpha family of membrane proteins are the central subunits of the putative protein translocation channel. We introduced random mutations into a segment of Escherichia coli SecY within its cytoplasmic domain 5, which was shown previously to be important for the SecA-dependent translocation activity. Mutations were classified into those retaining function and those gaining a dominant-interfering...
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