Functional consequences of insertions and deletions in the complementarity-determining regions of human antibodies.

Abstract excerpt

Insertions and deletions of nucleotides in the genes encoding the variable domains of antibodies are natural components of the hypermutation process, which may expand the available repertoire of hypervariable loop lengths and conformations. Although insertion of amino acids has also been utilized in antibody engineering, little is known about the functional consequences of such modifications. To investigate this...

Topics

• Amino Acid Sequence
• Antibodies
• Antibody Diversity
• Binding Sites, Antibody
• Circular Dichroism
• Complementarity Determining Regions
• Genes, Immunoglobulin
• Humans
• Immunoglobulin Fragments
• Models, Molecular
• Molecular Sequence Data
• Mutation
• Protein Structure, Tertiary
• Sequence Alignment