Article

<i>In vitro</i> Evolution of Antibody Affinity via Insertional Mutagenesis Scanning of an Entire Antibody Variable Region

2020-04-28

Abstract excerpt

We report the first systematic combinatorial exploration of affinity enhancement of antibodies by insertions and deletions (InDels). Transposon-based introduction of InDels via the method TRIAD was used to generate large libraries with random in-frame InDels across the entire scFv gene that were further recombined and screened by ribosome display. Knowledge of potential insertion points from TRIAD libraries formed...

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Literature Corpus work: 37bba2d9-df48-5dc1-b5bb-1690576a2a6b
DOI: 10.1101/2020.04.26.062786

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