Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis

Abstract excerpt

The identification of mutations that enhance antibody affinity while maintaining high antibody specificity and stability is a time-consuming and laborious process. Here we report an efficient methodology for systematically and rapidly enhancing the affinity of antibody variable domains while maximizing specificity and stability using novel synthetic antibody libraries. Our approach first uses computational and...

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