Article
[Comparative studies of serological typing and HLA-A, B antigen genotyping with PCR using sequence-specific primers].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Nov 2004
Wu Da-lin, Ling Han-xin, Tang Hao
Abstract excerpt
OBJECTIVE: To evaluate the accuracy of PCR with sequence-specific primers (PCR-SSP) for HLA-I genotyping and analyze the causes of the errors occurring in the genotyping. METHODS: DNA samples and were obtained from 34 clinical patients, and serological typing with monoclonal antibody (mAb) and HLA-A and, B antigen genotyping with PCR-SSP were performed. RESULTS: HLA-A and, B alleles were successfully typed in 34...
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