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[Establishment of an assay for cloning and sequencing the full-length HLA-Cw gene].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Jun 2009

Deng Zhi-hui, Xu Yun-ping, Gao Su-qing, Li Da-cheng, Yu Qiong, Su Yu-qing, Zeng Jian-qiang, Yang Bao-cheng

Abstract excerpt

OBJECTIVE: To establish a reliable assay for cloning and sequencing the full-length HLA-Cw gene. METHODS: In this study, a fragment of 4.5 kb full-length HLA-Cw gene was amplified using the self-designed PCR primer pair by long template PCR, purified PCR products was cloned into the pGEM-Teasy plasmid vector and the plasmid DNA isolated from positive clones was subjected to haplotype sequencing by both...

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Identifiers

PMID: 19504435
DOI: 10.3760/cma.j.issn.1003-9406.2009.03.005

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