Article
[Construction and expression of hybrid genes based on a deletion mutant of the herpes virus thymidine kinase gene in Escherichia coli].
Molekuliarnaia biologiia - 1 Jan 2000
Zemskova M Iu, Dzhavakhishvili Ts D, Bukhtiiarova M G, Berezina Iu V, Fodor I I
Abstract excerpt
The plasmids with the deletion mutant of thymidine kinase gene (tk') of Herpes simplex virus were constructed. The promoter, transcription initiation site and first 35 codons of tk gene were removed and replaced with a series of DNA restriction sites. The DNA fragments, containing the gene regula...
Topics
- Autoradiography
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Viral
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids