Article

A new dual prokaryotic ( <i>E. coli</i> ) and mammalian expression system (pgMAXs)

2020-05-21

Abstract excerpt

We introduce an efficient subcloning and expression plasmid system with two different modes (prokaryotic for expression in Escherichia coli with lac promoter and mammalian modes with cytomegalovirus promoter). The efficient subcloning (DNA insertion) is based upon a DNA topoisomerase II toxin-originated gene for effective selection with isopropyl-β-D-thiogalactoside (IPTG) induction. The new pgMAXs system is mana...

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Literature Corpus work: a988caee-b079-5e56-8176-458c7fde8af4
DOI: 10.1101/2020.05.21.108126

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