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Article

Rapid carrier screening for beta-thalassemia by single-step allele-specific PCR and detection.

Clinical biochemistry - 1 Mar 2007

Quek Dollyn Liying, Ng Yen-Yek, Wang Wen, Tan Arnold S C, Tang-Lim Guek-Im, Ng Ivy S L, Law Hai-Yang, Chong Samuel S

Abstract excerpt

OBJECTIVES: This study aimed to evaluate a rapid molecular carrier screening strategy for beta-thalassemia. DESIGN AND METHODS: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR Green I fluorescence in a single step. RESULTS: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each...

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Identifiers

PMID: 17296174
DOI: 10.1016/j.clinbiochem.2007.01.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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