Article
High-throughput genotyping with energy transfer-labeled primers.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Khripin Yuri
Abstract excerpt
The Amplifluor method for single-nucleotide polymorphisms (SNP) genotyping provides homogeneous assays that utilize a pair of universal energy transfer-labeled primers. The main advantage of this single-step, loci-independent, low-cost method is that it can be readily adapted for new SNPs. The development of any new SNP assay requires only the design and synthesis of three conventional oligonucleotides....
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