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Article

Subdomains in the F and G helices of bacteriorhodopsin regulate the conformational transitions of the reprotonation mechanism.

Proteins - 1 Aug 2002

Martinez Lynell C, Thurmond Robin L, Jones Philip G, Turner George J

Abstract excerpt

We have performed cysteine scanning mutagenesis of the bacteriorhodopsin mutant D85N to explore the role of individual amino acids in the conformational transitions of the reprotonation mechanism. We have used whole-cell reflectance spectroscopy to evaluate the spectral properties of the 59 mutants generated during a scan of the entire F and G helices and the intervening loop region. Cys mutants were grouped into...

Topics

Amino Acid Sequence
Bacteriorhodopsins
Biological Transport
Cysteine
Hydrogen-Ion Concentration
Lipid Metabolism
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Protein Structure, Secondary

Protons

Source

Identifiers

PMID: 12112695
DOI: 10.1002/prot.10158

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