High-throughput screening of bacteriorhodopsin mutants in whole cell pastes.

doi:10.1016/s0005-2736(02)00406-6

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Article

High-throughput screening of bacteriorhodopsin mutants in whole cell pastes.

Biochimica et biophysica acta - 19 Aug 2002

Martinez Lynell C, Turner George J

Abstract excerpt

A high-throughput screening method has been developed which enables functional analysis of bacteriorhodpsin in whole cell pastes. Reflectance spectra, from as little as 5 ml of Halobacterium salinarum cells, show close correspondence to that obtained from the purified purple membrane (PM), containing bacteriorhodopsin (BR) as the sole protein component. We demonstrate accurate quantification of BR accumulation by...

Topics

Bacteriorhodopsins
Halobacterium salinarum
Hydrogen-Ion Concentration
Mutagenesis, Site-Directed
Mutation
Protein Conformation
Purple Membrane
Recombinant Proteins
Spectrophotometry

Source

Open in PubMed

Identifiers

PMID: 12101000
DOI: 10.1016/s0005-2736(02)00406-6

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