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Article

Generation and analysis of bacteriorhodopsin mutants with the potential for biotechnological applications.

Bioengineered - 1 Jan 2000

Saeedi P, Moosaabadi J Mohammadian, Sebtahmadi S Sina, Mehrabadi J Fallah, Behmanesh M, Nejad H Rouhani, Nazaktabar A

Abstract excerpt

The properties of bacteriorhodopsin (BR) can be manipulated by genetic engineering. Therefore, by the methods of gene engineering, Asp85 was replaced individually by two other amino acids (D85V, D85S). The resulting recombinant proteins were assembled into soybean vesicles retinylated to form functional BR-like nano-particles. Proton translocation was almost completely abrogated by the mutant D85S, while the D85V...

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Identifiers

PMID: 22976247
DOI: 10.4161/bioe.21048
PMCID: PMC3477695

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Source receipt: pubmed, retrieved 7 Sept 2026.

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