Article
A redirected proton pathway in the bacteriorhodopsin mutant Tyr-57-->Asp. Evidence for proton translocation without Schiff base deprotonation.
The Journal of biological chemistry - 18 Nov 1994
Sonar S, Marti T, Rath P, Fischer W, Coleman M, Nilsson A, Khorana H G, Rothschild K J
Abstract excerpt
Light-driven proton pumping in bacteriorhodopsin involves deprotonation of the retinylidene Schiff base during M formation and reprotonation during N formation as key steps. This study reports on the spectroscopic characterization of the bacteriorhodopsin mutant Tyr-57-->Asp (Y57D). The results reveal that although formation of the M intermediate and Schiff base deprotonation is blocked, the mutant still exhibits...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.