Article
Non-essential roles of cysteine residues in functional expression and redox regulatory pathways for canine glutamate/aspartate transporter based on mutagenic analysis.
The Biochemical journal - 1 Oct 2002
Tamahara Satoshi, Inaba Mutsumi, Sato Kota, Matsuki Naoaki, Hikasa Yoshiaki, Ono Ken-Ichiro
Abstract excerpt
A redox regulatory mechanism and a molecular link between oxidative and excitotoxic neurodegeneration have been postulated for high-affinity Na(+)-dependent glutamate transporters. In the present study, mutations were introduced at three cysteine residues in canine glutamate/aspartate transporter (GLAST) to investigate the functional significance of thiol groups in response to oxidation. Cys(-) GLAST, in which...
Topics
- Amino Acid Transport System X-AG
- Animals
- Biological Transport
- COS Cells
- Cell Membrane
- Cloning, Molecular
- Cysteine
- DNA, Complementary
- Dithiothreitol
- Dogs
- Glutamic Acid
- Kinetics
- Mercury