Article
Abolition of substrate-dependent currents by tyrosine mutation in the transmembrane domain of glutamate transporter.
FEBS letters - 24 Mar 1997
Choi I, Chiu S Y
Abstract excerpt
By site-directed mutagenesis we examined the roles of tyrosine residues (Tyr127) in the putative transmembrane domain of rat glutamate transporter (GLAST). When expressed in Xenopus oocytes, Y127F mutant protein, which was localized in plasma membranes of oocytes, completely abolished glutamate u...
Topics
- Amino Acid Transport System X-AG
- Animals
- Biological Transport
- Carrier Proteins
- Electric Conductivity
- Electrophysiology
- Glutamic Acid
- Glycoproteins
- Immunohistochemistry
- Membrane Glycoproteins
- Mutagenesis, Site-Directed
- Mutation
- Oocytes
- Rats
- Recombinant Proteins
- Tyrosine
- Xenopus