Article
Computer-aided design of a PDZ domain to recognize new target sequences.
Nature structural biology - 1 Aug 2002
Reina Jose, Lacroix Emmanuel, Hobson Scott D, Fernandez-Ballester Gregorio, Rybin Vladimir, Schwab Markus S, Serrano Luis, Gonzalez Cayetano
Abstract excerpt
PDZ domains are small globular domains that recognize the last 4-7 amino acids at the C-terminus of target proteins. The specificity of the PDZ-ligand recognition is due to side chain-side chain interactions, as well as the positioning of an alpha-helix involved in ligand binding. We have used computer-aided protein design to produce mutant versions of a Class I PDZ domain that bind to novel Class I and Class II...
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