Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.

Journal of molecular biology - 19 Apr 2002

Kaufmann Markus, Lindner Peter, Honegger Annemarie, Blank Kerstin, Tschopp Markus, Capitani Guido, Plückthun Andreas, Grütter Markus G

Abstract excerpt

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydrogen bonds to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alkaline Phosphatase
Share to Antibody Affinity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Antibody Specificity

Share to Antigen-Antibody Complex

Share to Crystallization

Share to Immunoglobulin Fragments

Share to Sequence Tagged Sites

Share to X-Ray Diffraction

Source

Identifiers

PMID: 12054774
DOI: 10.1016/S0022-2836(02)00038-4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.PMID 12054774Read ↗

Characterization and molecular modeling of a highly stable anti-Hepatitis B surface antigen scFv.Semantic proximity Read ↗

Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies.Semantic proximity Read ↗

Improved antigen binding by a CD20-specific single-chain antibody fragment with a mutation in CDRH1.Semantic proximity Read ↗

A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.Semantic proximity Read ↗

Strategies to improve scFvs as crystallization chaperones suggested by analysis of a complex with the human PHD-bromodomain SP140Semantic proximity Read ↗

Crystallization and diffraction properties of the Fab fragment of 3B5H10, an antibody specific for disease-causing polyglutamine stretches.Semantic proximity Read ↗

Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity.Semantic proximity Read ↗

Crystal structure of a human CD3-ε/δ dimer in complex with a UCHT1 single-chain antibody fragmentSemantic proximity Read ↗

Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody.Semantic proximity Read ↗

High-resolution structure of bovine pancreatic trypsin inhibitor with altered binding loop sequence.Semantic proximity Read ↗

The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.Semantic proximity Read ↗

Removal of the conserved disulfide bridges from the scFv fragment of an antibody: effects on folding kinetics and aggregation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.