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Article

A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.

Protein science : a publication of the Protein Society - 1 Oct 2005

Honegger Annemarie, Spinelli Silvia, Cambillau Christian, Plückthun Andreas

Abstract excerpt

The structure of the scFv fragment FITC-E2, obtained from a naive phage antibody scFv library derived from human donors, was determined at 2.1 A resolution in the free form and at 3.0 A in the complexed form. The wild-type (wt) scFv binds fluorescein with a K(D) of 0.75 nM. The free scFv readily crystallizes by compacting its 18 amino acid-long CDR-H3, partially occluding the binding site and further blocking...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 16195545
DOI: 10.1110/ps.051520605
PMCID: PMC2253284

Genes

AFM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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