Site-specific recombination in eukaryotic cells mediated by mutant lambda integrases: implications for synaptic complex formation and the reactivity of episomal DNA segments.

Abstract excerpt

Mutant lambda integrases catalyze site-specific DNA recombination in the absence of accessory factors IHF, XIS, and negative DNA supercoiling. Here we investigate the effects that a human cellular environment exerts on these reactions in order to (i) gain further insights into mechanistic aspects of recombination in eukaryotic cells and (ii) to further develop the Int system for biotechnological applications....

Topics

• Attachment Sites, Microbiological
• Bacterial Proteins
• Bacteriophage lambda
• Binding Sites
• Chromosome Pairing
• Eukaryotic Cells
• Flow Cytometry
• HeLa Cells
• Humans
• Integrases
• Integration Host Factors
• Kinetics
• Mutation
• Plasmids