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Article

Site-specific recombination in mammalian cells catalyzed by gammadelta resolvase mutants: implications for the topology of episomal DNA.

FEBS letters - 14 Apr 2000

Schwikardi M, Dröge P

Abstract excerpt

We have transferred the prokaryotic gammadelta resolvase system to mammalian cells and present a comparative analysis of recombination by wild-type and two mutant resolvases (E124Q and E102Y/E124Q). Transient co-transfection assays using beta-galactosidase as reporter for recombination reveal that episomal DNA does not contain a significant level of unconstrained negative supercoiling, since only mutant...

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PMID: 10767411
DOI: 10.1016/s0014-5793(00)01394-6

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