Article
The RecJ DNase strongly suppresses genomic integration of short but not long foreign DNA fragments by homology-facilitated illegitimate recombination during transformation of Acinetobacter baylyi.
Molecular microbiology - 1 May 2007
Harms Klaus, Schön Verena, Kickstein Eva, Wackernagel Wilfried
Abstract excerpt
Homology-facilitated illegitimate recombination (HFIR) promotes genomic integration of foreign DNA with a single segment homologous to the recipient genome by homologous recombination in the segment accompanied by illegitimate fusion of the heterologous sequence. During natural transformation of Acinetobacter baylyi HFIR occurs at about 0.01% of the frequency of fully homologous recombination. The role of the 5'...
Topics
- Acinetobacter
- Bacterial Proteins
- DNA, Bacterial
- Exodeoxyribonucleases
- Genome, Bacterial
- Models, Genetic
- Mutation
- Nucleic Acid Heteroduplexes
- Plasmids
- Polymerase Chain Reaction
- Recombination, Genetic
- Transformation, Bacterial