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Article

Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase.

Journal of molecular biology - 25 Aug 2000

Declerck N, Machius M, Wiegand G, Huber R, Gaillardin C

Abstract excerpt

Bacillus licheniformis alpha-amylase (BLA) is a starch-degrading enzyme that is highly thermostable although it is produced by a rather mesophilic organism. Over the last decade, the origin of BLA thermal properties has been extensively investigated in both academic and industrial laboratories, yet it is poorly understood. Here, we have used structure-based mutagenesis in order to probe the role of amino acid...

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Identifiers

PMID: 10966804
DOI: 10.1006/jmbi.2000.4025

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