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Article

Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases.

European journal of biochemistry - 1 Sept 1997

Veltman O R, Vriend G, Hardy F, Mansfeld J, van den Burg B, Venema G, Eijsink V G

Abstract excerpt

Site-directed mutagenesis was used to assess the contribution of individual residues and a bound calcium in the 55-69 region of the thermolysin-like protease of Bacillus stearothermophilus (TLP-ste) to thermal stability. The importance of the 55-69 region was reflected by finding that almost all mutations had drastic effects on stability. These effects (both stabilizing and destabilizing) were obtained by...

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Identifiers

PMID: 9346299
DOI: 10.1111/j.1432-1033.1997.00433.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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