Article
Selective inhibition of the C-domain of angiotensin I converting enzyme by bradykinin potentiating peptides.
Biochemistry - 14 May 2002
Cotton Joël, Hayashi Mirian A F, Cuniasse Philippe, Vazeux Gilles, Ianzer Danielle, De Camargo Antonio C M, Dive Vincent
Abstract excerpt
Somatic angiotensin I converting enzyme (ACE) contains two functional active sites. Up to now, most of the studies aimed at characterizing the selectivity of inhibitors toward the two ACE active sites relied on the use of ACE mutants containing a single functional active site. By developing new fluorogenic synthetic substrates of ACE, we demonstrated that inhibitor selectivity can be assessed directly by using...
Topics
- Amino Acid Sequence
- Angiotensin-Converting Enzyme Inhibitors
- Animals
- Bradykinin
- CHO Cells
- Catalytic Domain
- Cricetinae
- Fluorescent Dyes
- Humans
- In Vitro Techniques
- Kinetics
- Lisinopril
- Models, Biological
- Mutation