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Article

Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation.

Nature biotechnology - 1 May 2002

Eggan Kevin, Rode Anja, Jentsch Isabell, Samuel Caroline, Hennek Thomas, Tintrup Hartmut, Zevnik Branko, Erwin Jennifer, Loring Janet, Jackson-Grusby Laurie, Speicher Michael R, Kuehn Ralf, Jaenisch Rudolf

Abstract excerpt

We have devised a general strategy for producing female mice from 39,X0 embryonic stem (ES) cells derived from male cell lines carrying a targeted mutation of interest. We show that the Y chromosome is lost in 2% of subclones from 40,XY ES cell lines, making the identification of targeted 39,X0 subclones a routine procedure. After gene targeting, male and female mice carrying the mutation can be generated by...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 11981557
DOI: 10.1038/nbt0502-455

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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