Article
Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore.
Nucleic acids research - 1 May 2002
Nazarenko Irina, Lowe Brian, Darfler Marlene, Ikonomi Pranvera, Schuster David, Rashtchian Ayoub
Abstract excerpt
Multiplex quantitative PCR based on novel design of fluorescent primers is described. Fluorogenic primers are labeled with a single fluorophore on a base close to the 3' end with no quencher required. A tail of 5-7 nt is added to the 5' end of the primer to form a blunt-end hairpin when the primer is not incorporated into a PCR product. This design provides a low initial fluorescence of the primers that increases...
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