Article
Sensitive single-stage PCR using custom-synthesized internal controls.
BioTechniques - 1 Apr 2000
Zimmermann K, Rieger M, Gross P, Turecek P L, Schwarz H P
Abstract excerpt
A new approach for an internally controlled PCR was developed using a custom-synthesized oligonucleotide as the internal control. Three different PCR setups demonstrated the usefulness of this approach: (i) the addition of the respective internal control to samples containing ssDNA virus Parvo B19; (ii) the co-extraction of plasma samples and the respective internal control for the detection of the ssDNA virus...
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