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Multiplex real-time single nucleotide polymorphism detection and quantification by quencher extension.

BioTechniques - 1 Mar 2006

Rudi Knut, Zimonja Monika, Hannevik Sigrun E, Drømtorp Signe M

Abstract excerpt

Multiplex quencher extension (multiplex-QEXT) is a novel closed tube single-step method for detection and quantification of several single nucleotide polymorphisms (SNPs) simultaneously. The principle of multiplex-QEXT is that 5' reporter-labeled probes are 3' single-base-extended with TAMRA dideoxy nucleotides if the respective SNP alleles are present. TAMRA can serve as either an energy acceptor (quencher-based...

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PMID: 16568821
DOI: 10.2144/000112118

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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