Article
The use of real-time PCR and fluorogenic probes for rapid and accurate genotyping of newborn mice.
Molecular and cellular probes - 1 Jun 2001
Hnatyszyn H J, Podack E R, Young A K, Seivright R, Spruill G, Kraus G
Abstract excerpt
Real-time PCR and fluorogenic probes were combined in a simple, rapid and sensitive method to genotype murine breeding stocks and their progeny for a point mutation. DNA from tail biopsies of newborn mice was mixed with amplification primers and fluorogenic hybridization probes in a PCR mixture. The primers were designed to amplify a region of the Fas-Ligand gene including the site for the gld natural point...
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